JCECE Medical JCECE Medical Solved Paper-2015

  • question_answer
    Which of the given statement is correct in the context of observing DNA separation by agarose gel electrophoresis?

    A)  DNA can be seen in visible light.

    B)  DNA can be seen without staining in visible light.

    C)  Ethidium bromide stained DNA can be seen in visible light.

    D)  Ethidium bromide stained DNA can be seen under exposure to UV light.

    Correct Answer: D

    Solution :

     The separated DNA molecules are visualised only after staining DNA with ethidium bromide followed by exposure to UV radiation. The appear as bright orange coloured bands. The separated bands of DNA (on the gel) are cut from the agarose gel and extracted from the gel piece.


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