Uttarakhand PMT Uttarakhand PMT Solved Paper-2010

  • question_answer
    A student wishes to study the cells structure under a light microscope having 10X eyepiece and 45X objective. He should illuminate the object by which one of the following colors of light so as to get the best possible resolution?

    A)  Yellow          

    B)  Green

    C)  Blue            

    D)  Red

    Correct Answer: C

    Solution :

    Resolving power or resolution is the ability of the lens to distinguish fine details and structure. Specifically, it refers to the ability of the lenses to distinguish between two points at a specr- distance apart. Resolving power depends on r- factors : (i) Wavelength of light used for illumination (ii) Power of objective lenses. \[\text{Resolving power =}\frac{\text{Wavelength of light}}{\text{2 }\!\!\times\!\!\text{ NA}}\] Since, the limit of resolving power  of microscoot is fixed by the structure of light, the shortest wavelength of visible light will give the maximum resolutions. Among yellow, green, red and blue light colour, blue colour (500 nm) have shortest wavelength so, it will give best resolution.


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