Answer:
RegionA Barn HI
RegionB Pot I
Region C ampR
E. coli cloning vector pBR322
showing restriction sites (Hind III, Eco Rl, Bam HI, SalI,
Pvu II, Pst I, Cla I), Ori and antibiotic
resistance genes (ampR and tetR).
Rop codes for the proteins
involved in the replication of the plasmid.
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