Answer:
When the symptoms of the disease are not
yet visible, the pathogen concentration is very low so, the detection by
conventional diagnostic tests is very difficult. However, detection of a
disease before the appearance of symptoms is possible by the amplification of
victim's nucleic acid by PCR .The principle involved here is that a single DNA
molecule can be copied endlessly in a test tube using primers, DNA polymerase
enzyme and free nucleotides. The desired DMA of the pathogen is amplified by
PCR from a limited amount of DNA template.
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