(c) In E. coil (bacterium, the RNA
polymerase has co-factors p, [3', a, a' and co along with(sigma) factor, to catalyze
The transcription is completed in three
steps. Initiation o (sigma) factor recognizes the start signal and promoter
region on DNA which then along with RNA polymerase binds to the promoter to
Elongation The RNA
polymerase after initiation of RNA transcription loses the factor but
continues the polymerization of rib nucleotides to form RNA,
Termination Once the RNA
polymerase reaches the termination region of DNA, the
RNA polymerase is separated from
DNA-RNA hybrid, as a result nascent RNA separates. This process is called
termination which is facilitated by a termination factor(rho).
In prokaryotes, mRNA does not require
any processing, so both transcription and translation occur in the cytosol. It
can be said that transcription and translation are coupled together.
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