Use of 15N will be inappropriate
because method of detection of 32P and 15N different
(32P being a radioactive
isotope while15N is non-radioactive but is the heavier isotope of nitrogen).
Even if15N was
radioactive then its presence would have been detected, both inside the cell(15N
incorporated as introgenous base in DNA) as well as in the supernatant, because15N
would also get incorporated in amino group of amino acids in proteins. Hence,
the use of 15N would not give any conclusive results.
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