NEET Biology Biotechnology : Principles And Processes Question Bank

done Self Evaluation Test - Biotechnology: Principles and Processes Total Questions - 42

• question_answer1)
  Restriction endonucleases:
  A)
  are enzymes that process pre-RNA. done clear
  B)
  are enzymes that degrade DNA. done clear
  C)
  protect bacterial cells from viral infection. done clear
  D)
  None of the above. done clear
  View Solution play_arrow

• question_answer2)
  A gene is said to be cloned if:
  A)
  the DNA sequence of the gene is known. done clear
  B)
  the function of the gene is known. done clear
  C)
  there is a DNA probe for the gene. done clear
  D)
  the gene has been isolated and copied. done clear
  View Solution play_arrow

• question_answer3)
  Which of the following is a critically important tool used in experiments involving DNA hybridisation?
  A)
  DNA sequencing machines done clear
  B)
  Ligases done clear
  C)
  DNA probes done clear
  D)
  Vectors done clear
  View Solution play_arrow

• question_answer4)
  Introduction of one or more genes into an organism which normally does not possess them or their deletion by using artificial mean (not by breeding) comes under:
  A)
  Molecular biology done clear
  B)
                     Cytogenetics done clear
  C)
  Genetic hybridization                   done clear
  D)
  Genetic engineering done clear
  View Solution play_arrow

• question_answer5)
  A genetic marker is a:        
  A)
  place where a restriction enzyme cuts DNA. done clear
  B)
  chart that traces the family history of a genetic trait. done clear
  C)
  nucleotide sequence near a particular gene. done clear
  D)
  radioactive probe used to find a gene. done clear
  View Solution play_arrow

• question_answer6)
  A nucleic acid probe might be used to:
  A)
  insert genes into a host cell. done clear
  B)
  make DNA for gene cloning. done clear
  C)
  splice pieces of DNA. done clear
  D)
  identify complementary segments present in nucleic acid sequence. done clear
  View Solution play_arrow

• question_answer7)
  DNA fragments with sticky ends are not allowed to undergo self ligation by:
  A)
  Unwindase done clear
  B)
  Single strand binding proteins done clear
  C)
  Gyrase done clear
  D)
  Alkaline phosphatase done clear
  View Solution play_arrow

• question_answer8)
  In genetic engineering, a chimera is:
  A)
  an enzyme that links DNA molecules. done clear
  B)
  a pasmid that contains foreign DNA. done clear
  C)
  a virus that infects bacteria. done clear
  D)
  a fungi. done clear
  View Solution play_arrow

• question_answer9)
  Restriction enzymes are used in genetic engineering because:
  A)
  they can join different DNA fragments. done clear
  B)
  they can cleave DNA at a specific target. done clear
  C)
  they are nucleases that cut DNA at variable sites. done clear
  D)
  they are proteolytic enzymes which can degrade harmful enzymes. done clear
  View Solution play_arrow

• question_answer10)
  Which of the following is known as specific molecular scissors?
  A)
  Ligase done clear
  B)
  Helicase done clear
  C)
  Restriction endonuclease done clear
  D)
  DNA polymerase done clear
  View Solution play_arrow

• question_answer11)
  The RTF region enables the plasmid to:
  A)
  be transmitted to otherbacteria by conjugation. done clear
  B)
  undergo transformation. done clear
  C)
  replicate in the host cell. done clear
  D)
  code for enzymes that inactivate specific drugs. done clear
  View Solution play_arrow

• question_answer12)
  Which one of the following option is correct for 1, 2, 3 and 4?           
  A)
  1-Exonuclease; 2-Endonuclease; 3-DNA ligase; 4-Transformation done clear
  B)
  1-Endonuclease; 2-Exonuclease; 3-DNA ligase; 4-Transformation done clear
  C)
  1-Exonuclease; 2-Endonuclease; 3- Hydrolase; 4-Transduction done clear
  D)
  1-Restriction endonuclease; 2-Restriction endonuclease; 3-DNA ligase; 4-Transformation done clear
  View Solution play_arrow

• question_answer13)
  Match column-I with column-II and choose the correct option.

  	Column-I		Column-II
  A.	Eco RI	I.	Bacillus amyloliquefaciens
  B.	Bam HI	II.	Haemophilus influenza
  C.	Hind III	III.	Escherichia coli
  D.	pBR 322	IV.	Artificial plasmid

  A)
  A-III;     B-I;     C-II;     D-IV done clear
  B)
  A-II;      B-I;     C-IV;    D-III done clear
  C)
  A-IV;     B-I;     C-III;    D-II done clear
  D)
  A-I;       B-IV;   C-II;     D-III done clear
  View Solution play_arrow

• question_answer14)
  Temperature required for various steps of polymerase chain reaction (i), (ii) and (iii) are:

  (i)   Denaturation                        (ii) Annealing

  (iii) Extension

  A)
  (i) \[-94{}^\circ C\], (ii) \[-60{}^\circ C\], (iii) \[-72{}^\circ C\] done clear
  B)
  (i)\[-40{}^\circ C\], (ii) \[-72{}^\circ C\], (iii) \[-94{}^\circ C\] done clear
  C)
  (i) \[-72{}^\circ C\] (ii) - 94°C, (iii) \[-40{}^\circ C\] done clear
  D)
  (i) \[-90{}^\circ C\], (ii) \[-72{}^\circ C\], (iii) \[-40{}^\circ C\] done clear
  View Solution play_arrow

• question_answer15)
  A \[{{T}_{4}}\] bacteriophage has a gene for the enzyme, lysozyme. The function of this enzyme is to digest:
  A)
   Cell wall     done clear
  B)
                     Cell membrane done clear
  C)
   Golgi                                        done clear
  D)
  Plasmid done clear
  View Solution play_arrow

• question_answer16)
  Most sensitive technique to detect malignant cell in non-Hodgkin's lymphoma is:
  A)
  Polymerase chain reaction done clear
  B)
  Gene therapy    done clear
  C)
   Stem cell therapy done clear
  D)
  None of the above done clear
  View Solution play_arrow

• question_answer17)
  In microinjection method, the DNA is incorporated into host cell by using:
  A)
  Micro-pipettes done clear
  B)
  Micro-needles done clear
  C)
  High voltage electric impulse done clear
  D)
  Both [a] and [b] done clear
  View Solution play_arrow

• question_answer18)
  Which of the following statement is incorrect?
  A)
  Mobile genetic elements, tranposons were visualised by Barbara McClintock. done clear
  B)
  Udder cell and somatic cell is used to produce the cloned sheep by nuclear transplantation method. done clear
  C)
  In pedigree analysis, a person immediately affected by and action is called propositus. done clear
  D)
  DNA ligases are used to cleave a DNA molecule. done clear
  View Solution play_arrow

• question_answer19)
  Which one of the following pairs of terms/names means one and the same thing?
  A)
  Gene pool of an organism-genome done clear
  B)
  Codon-gene done clear
  C)
  Cistron-triplet done clear
  D)
  DNA fingerprinting-DNA profiling done clear
  View Solution play_arrow

• question_answer20)
  Match column-I with column-II and select the correct answer using the codes given below.

  	Column-I		Column-II
  A.	Southern blotting	I.	Running of DNA fragments on get.
  B.	Gel electrophoresis	II.	Cutting of DNA into fragments.
  C.	Cleavage	III.	Transfer of DNA
  D.	DNA probing	IV.	Searching for desired DNA fragments

  A)
  A-IV;    B-I;      C-II;   D-III done clear
  B)
  A-III;     B-I;     C-II;    D-IV done clear
  C)
  A-IV;     B-I;     C-III;    D-II done clear
  D)
  A-I;       B-IV;   C-II;    D-III done clear
  View Solution play_arrow

• question_answer21)
  Polyethylene glycol method is used for:
  A)
  biodiesel production. done clear
  B)
  seedless fruit production. done clear
  C)
  energy production from sewage. done clear
  D)
  gene transfer without a vector. done clear
  View Solution play_arrow

• question_answer22)
  Plasmids are suitable vectors for gene cloning because:
  A)
  these are small circular DNA molecule which can integrate with host chromosomal DNA. done clear
  B)
  these are small circular DNA molecules with their own replication origin site. done clear
  C)
  these can shuttle between prokaryotic and eukaryotic cells. done clear
  D)
  these often carry antibiotic resistance genes. done clear
  View Solution play_arrow

• question_answer23)
  Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using:
  A)
  BACs                                        done clear
  B)
  Expression vectors done clear
  C)
  YACs                                        done clear
  D)
  All of these done clear
  View Solution play_arrow

• question_answer24)
  Which of the following is not a genetic vector?
  A)
  Plasmid           done clear
  B)
                     Phage done clear
  C)
  Cosmid          done clear
  D)
                     Virusoid done clear
  View Solution play_arrow

• question_answer25)
  The introduction of TDA into plants involves:
  A)
  exposing the plants to cold for a brief period. done clear
  B)
  allowing the plant roots to stand in water. done clear
  C)
  infection of the plant by Agrobacterium tumefaciens. done clear
  D)
  None of these done clear
  View Solution play_arrow

• question_answer26)
  Due to chloramphenicol resistance gene, one is able to select a transformed cell in the presence of chloramphenicol. The chloramphenicol resistance gene in this case is called:
  A)
  Origin of replication                     done clear
  B)
  Cloning sites done clear
  C)
  Selectable marker                        done clear
  D)
  None of the above done clear
  View Solution play_arrow

• question_answer27)
  Electrophoresis is used to:
  A)
  separate fragments of DNA. done clear
  B)
  clone genes. done clear
  C)
  cut DNA into fragments. done clear
  D)
  match a gene with its function. done clear
  View Solution play_arrow

• question_answer28)
  A biologist intends to use a polymerase chain reaction to perform a genetic task. The biologist probably is trying to:
  A)
  discover new genes. done clear
  B)
  clone a gene. done clear
  C)
  cut DNA into many small fragments. done clear
  D)
  isolate DNA from a living cell. done clear
  View Solution play_arrow

• question_answer29)
  Consider the given figure and identify the correct option.
  A)
  A - Denaturation, B - Annealing, C - Extension, D ? Amplified done clear
  B)
  A - Annealing, B - Deoaturation, C - Extension, D ? Amplified done clear
  C)
  A- Denaturation, B -Annealing, C -Amplified, D ? Extension done clear
  D)
  A-Annealing, B - Denaturation, C -Amplified, D ? Extension done clear
  View Solution play_arrow

• question_answer30)
  Which one of the following pairs is not correctly matched?
  A)
  Plasmid - Small piece of extra chromosomal DNA in bacteria done clear
  B)
  Interferon - An enzyme that interferes with DNA replication done clear
  C)
  Cosmid - A vector for carrying large DNA fragments into host cells done clear
  D)
  Myeloma - Antibody - producing tumour cells done clear
  View Solution play_arrow

• question_answer31)
  Which of the following is/are related to biotechnology?

  (i) In vitro fertilisation.

  (ii) Synthesis of a gene.

  (iii) Correcting a defective gene.

  (iv) Developing a DNA vaccine.

  A)
  (i) and (ii)        done clear
  B)
                     (ii) and (iii) done clear
  C)
  (iii) and (iv)       done clear
  D)
                     (i), (ii), (iii) and (iv) done clear
  View Solution play_arrow

• question_answer32)
  Match column-I with column-II and choose the correct option.

  	Column-I		Column-II
  A.	Recombinant DNA	I.	Sea Weeds
  B.	Ethidium bromide	II.	DNA staining
  C.	Gel electrophoresis	III.	Plasmid DNA that has incorporated into human
  D.	Agarose	IV.	Process by which DNA fragments are separated based on their size

  A)
  A-III;    B-II;   C-IV;    D-I done clear
  B)
  A-III;   B-II;   C-I;      D-IV done clear
  C)
  A-II;    B-I;    C-IV;    D-III done clear
  D)
  A-III;    B-IV;   C-I;    D-II done clear
  View Solution play_arrow

• question_answer33)
  DNA fragments are separated using gel electrophoresis:
  A)
  because DNA is pulled through the gel toward the negative end of the field. done clear
  B)
  because larger DNA fragments move faster through the gel than smaller DNA fragments. done clear
  C)
  to identify and isolate DNA fragments. done clear
  D)
  to synthesise DNA for cloning. done clear
  View Solution play_arrow

• question_answer34)
  Complementary base pairing is important for:
  A)
  ligation reactions with blunt-end DNA molecules. done clear
  B)
  hybridization between DNA and transcription factors. done clear
  C)
  restriction endonucleases to cut cell walls. done clear
  D)
  synthesising cDNA molecules from mRNA templates.         done clear
  View Solution play_arrow

• question_answer35)
  In genetic engineering, genes can be inserted from one organism into another or back into the original organism by using which of the following techniques?
  A)
  Polymerase chain reaction done clear
  B)
  Gene gun done clear
  C)
  DNA hybridization done clear
  D)
  Gel electrophoresis done clear
  View Solution play_arrow

• question_answer36)
  A restriction fragment containing a specific gene of interest can be identified by gel electrophoresis followed by transferring the DNA to a membrane as solid support matrix using a procedure called:
  A)
  Gene amplification done clear
  B)
  Southern blotting done clear
  C)
  Identification of a gene done clear
  D)
  Me Clintock done clear
  View Solution play_arrow

• question_answer37)
  Restriction endonuclease - Hind. II always cuts DNA molecules at a particular point by recognising a specific sequence of:
  A)
  six base pairs     done clear
  B)
                     five base pairs done clear
  C)
  four base pairs    done clear
  D)
                     seven base pairs done clear
  View Solution play_arrow

• question_answer38)
  Which of the following is/are used in recombinant DNA technology?

  (i) Agarose gel

  (ii) Ethidium bromide

  (iii) Plasmid vector

  (iv) Restriction endonuclease

  A)
  (i) and (ii)        done clear
  B)
                     (ii) and (iii) done clear
  C)
  (iii) and (iv)                                done clear
  D)
  (i), (ii), (iii) and (iv) done clear
  View Solution play_arrow

• question_answer39)
  Which of the following statements about restriction enzymes is incorrect?
  A)
  They work on DNA extracted from all types of organisms. done clear
  B)
  They are used to glue short segments of DNA. done clear
  C)
  They come in many varieties, each with its own DNA target sequence. done clear
  D)
  They are highly specific for their DNA target sequences. done clear
  View Solution play_arrow

• question_answer40)
  Which of the following statements represent/s 'cloning'?                
  A)
  all of the cells are derived from one cell and are genetically identical. done clear
  B)
  a gene from one organism has been inserted into a vector and successfully introduced into a host cell. done clear
  C)
  all of the cells in a particular organism are identical. done clear
  D)
  All of the above done clear
  View Solution play_arrow

• question_answer41)
  Recombinant DNA can be transferred into host cell by:             
  A)
  growing the host cell in growth medium containing ampicillin. done clear
  B)
  coating the DNA with carbohydrates so that the cells will engulf the DNA. done clear
  C)
  treating cells with calcium ions or electrical pulses to increase cell permeability. done clear
  D)
  injecting proteins into host cells to make them more permeable. done clear
  View Solution play_arrow

• question_answer42)
  Identify the correct statements.

  (i) The first recombinant DNA was constructed by using a piece of DNA from a plasmid carrying antibiotic resistance gene in the bacterium Salmonella typhimurium and linked it to the plasmid of E. coli.

  (ii) Cohen and Boyer are known as father of genetic engineering.

  (iii) When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together using DNA ligases.

  (iv) Endonucleases remove nucleotides from the ends of the DNA whereas exonucleases make cuts at specific positions within the

  DNA.

  (v) Presence of more than one recognition sites within the vector will generate several fragments which will complicate the gene cloning.

  (vi) Humulin was the first recombinant DNA based product, produced and marketed in India.

  (vii) YAC vectors contain the telomeric sequence, the centromere and autonomously replicating sequence from yeast chromosomes.

  (viii)Alkaline phosphatase, is used to prevent unwanted self ligation of the vector DNA molecules in procedures of rDNA technology.

  (ix) pBR 322 vector was the first artificial ideal vector constructed by Boliver and Rodriguez.

  (x) Plasmid DNA is coated with histone proteins and can act as genetic factor.

  A)
  (ii), (iv), (vi) and (x) done clear
  B)
  (ii), (iv), (v) and (x) done clear
  C)
  (i), (iii), (v), (vii), (viii) and (ix) done clear
  D)
  (i), (ii), (iv), (vi) and (x) done clear
  View Solution play_arrow