question_answer

Kartik has identified and isolated a useful gene from a bacterium. Now, he wants to transfer this gene to a plant to get a transgenic plant. With the help of a flow chart, show different steps, which he should take for the same.

Answer:

After identifying a useful gene from a bacterium, Kartik should follow following steps

Isolation of useful gene using restriction endonucleases.

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Transferring the gene to a suitable vector to create a recombinant DNA molecule.

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Transfer these recombinant DNA molecule to the target cells.

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Screening of cells for transformation.

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Selection of transformed cells.

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Regeneration of plants from the transformed cells to get transgenic plants.