A) Binding of DNA to the cell wall with the help of proteins.
B) Uptake of DNA through membrane transport proteins.
C) Uptake of DNA through transient pores in the bacterial cell wall.
D) Expression of antibiotic resistance gene.
Correct Answer: C
Solution :
DNA being a hydrophilic molecule cannot pass through cell membranes. Therefore, the bacteria should be made competent to accept the DNA molecule. In this case, the cell is treated with specific concentration of a divalent cation such as calcium to increase pore size in cell wall. The cells are incubated with recombinant DNA on ice, followed by placing them briefly at \[42{}^\circ C\]and then putting them back on ice. This is called heat shock treatment. The bacterium now takes up the recombinant DNA.
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