NEET AIPMT SOLVED PAPER MAINS 2012

  • question_answer
    The figure below shows three steps (I, II, III) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents?                                                

    A)  B-Denaruration at a temperature of about \[98{}^\circ C\] separating the two DNA strands

    B)  A-benaturation at a temperature of about \[50{}^\circ C\]

    C)  C-Extension in the presence of heat stable DNA polymerase

    D)  A-Annealing with two sets of primers

    Correct Answer: C

    Solution :

    There are three steps in polymerase chain reaction namely. (i) Denaturation-at \[94{}^\circ C\] for DNA strand separation. (ii)Annealing-binding of primer. (iii) Extension in presence of DNA polymerase which is heat stable.


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