BCECE Medical BCECE Medical Solved Papers-2003

  • question_answer
    PCR was developed by :

    A)  Karry Mullis (1983)

    B)  E. Southern (1980)

    C)  Francis Collins (1985)

    D)  James D. Watson (1953)

    Correct Answer: A

    Solution :

    Karry Mullis (1983) discovered polymerase chain reaction, which is widely exploited in gene cloning for genetic engineering. He made use of a thermostable enzyme Taq DNA polymerase (acts as \[{{72}^{o}}C\]) isolated from Thermus aquaticus. E.M. Southern (1975) developed a method called, Southern blotting technique for analysing the related genes in a DNA restriction fragment. James D. Watson and F.H.C Crick (1953) proposed a model for DNA comprising of two helically intertwined chains tied together by hydrogen bonds between purines and pyrimidines.


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