A) B - lymphocytes and myeloma cells
B) lymphoma cells and bone marrow cells
C) T - lymphocytes and myeloma cells
D) B - lymphocytes and carcinoma cells
Correct Answer: A
Solution :
B-lymphocytes and myeloma (bone marrow cancer) cells Monoclonal antibodies (Mabs) are pure antibodies against a particular autigen, which are obtained through clonal culture of hybridomas (cells which secrete the desired mab). This technique was developed by Vohler and Miltstein (1974). Usually B-lymphocytes and myeloma cells are employed to form hybridomas, which are then cultured either (in vivo) in peritoneal (cavity of mice) or in vitro (in large culture vessels). Monoclonal antibodies today are proving to be highly important in fields of immunological research and molecular biology because of their high specificity. They are being used in human therapy (diagnosis, treatment and prevention), suppression of immune response (organ rejections), cancer therapy to identify destroy tumor cells), diagnosis of allergies, hormone tests (hCG), identification of specialized cells (e.g., having a particular antigen), preparation of vaccines, study of cell structure, identifying particular gene carrying chromosomes, purification of commercial protein mixture etc.
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