CET Karnataka Medical CET - Karnataka Medical Solved Paper-2006

  • question_answer
    Restriction endonucleases are most widely used in recombinant DNA technology. They are obtained from:

    A)  bacteriophages

    B)  bacterial cells

    C)  plasmids       

    D)  all prokaiyotic cells

    Correct Answer: B

    Solution :

    Endonucleases are enzymes that produce internal cuts called cleavage in DNA molecules. A class of endonucleases cleave DNA only within or near those sites which have   specific  base   sequences.   Such endonucleases are known as restriction endonuclease and the site recognised by them as recognition site. These are obtained from the bacterial cells. Restriction enzymes were discovered due to and named after the phenomenon of host restriction of bacterial phages. Plasmid is a DNA molecule other than bacterial chromosome that is capable of independent replication and transmission. These are used as vector in genetic engineering.


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