| I. Synthesis of gene (DNA) for human insulin artificially |
| II. Culturing recombinant E. coli in bioreactors |
| III. Purification of humulin |
| IV. Insertion of human insulin gene into plasmid |
| V. Introduction of recombinant plasmid into E. coli |
| VI. Extraction of recombinant gene product from E. coli |
A) I, IV, V, II, VI, III
B) III, V, II, I, VI, IV
C) II, I, IV, III, V, VI
D) I, III, V, VI, II, IV
Correct Answer: A
Solution :
The correct sequence of the steps involved in the production of humulin through recombinant DNA technology are| Synthesis of gene for human insulin artificially. |
| Insertion of human insulin gene into plasmid. |
| Introduction of recombinant plasmid into E. coli. |
| Culturing recombinant E. coli in bioreactors. |
| Extraction of recombinant gene product from E. coli. |
| Purification of humulin. |
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