CET Karnataka Medical CET - Karnataka Medical Solved Paper-2011

  • question_answer
    The term southern blotting refers to

    A)  transfer of DNA fragments from in vitro cellulose membrane to electrophoresis gel

    B)  attachment of probes to DNA fragments

    C)  transfer of DNA fragments  from electrophoresis gel to nitrocellulose sheet

    D)  comparison of DNA fragments from two sources

    Correct Answer: C

    Solution :

    Southern hybridization is DNA-DNA hybridization. It is also called southern blotting since the procedure for transfer of DNA from the electrophoresis gel to nitrocellulose filter resembles blotting.


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