A) before fertilization
B) after fertilization
C) at the time of cleavage
D) at the time of amphimixis.
Correct Answer: B
Solution :
: After fertilization, structural proteins derived from the contents of the cortical granules of egg harden the vitetline membrane and transform it into the fertilization membrane (envelope) or activation calyx and prevent the entry of other sperms. It is a slow method to prevent polyspermy.
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