A) antibiotic resistance gene
B) lac z promoter gene
C) \[\beta \]- galactosidase gene
D) ori site
Correct Answer: C
Solution :
: In the production of recombinant insulin, a recombinant DNA possessing the genes for a and P-polypeptides is inserted in the coding sequence of an enzyme (3-galactosidase. This causes inactivation of the enzyme which is called insertional inactivation. If the plasmid in the bacterium does not have an insert, the presence of a chromogenic substrate gives blue coloured colonies. Presence of insert results into insertional inactivation of the P-galactosidase and, therefore, the colonies do not produce any colour, these colonies are marked as recombinant colonies.
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