question_answer

What is the first step in the Southern blot technique ?

A)  Denaturation of DNA on the gel for hybridization with specific probe

B) Production of a group of genetically        identical cells

C)  Digestion of DNA by restriction enzyme

D)  Denaturation of DNA from a nucleated cell such as the one from the scene of crime

Correct Answer: C

Solution :

First step in the Southern blot technique is the digestion of DNA by restriction enzyme. Restriction fragment preparation Separation by electrophoresis Denaturation of DNA by chemical  treatment Single stranded DNA are transferred on to special paper or nylon Use of radioactive probe Autoradiography