A) Denaturation of DNA on the gel for hybridization with specific probe
B) Production of a group of genetically identical cells
C) Digestion of DNA by restriction enzyme
D) Isolation of DNA from a nucleated cell such as the one from the scene of crime
Correct Answer: D
Solution :
The first step in southern blot technique is isolation of DNA from a nucleated cell and then these isolated DNA sequences are transferred to a nylon or nitrocellulose sheet placed over the gel.
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