NEET AIPMT SOLVED PAPER MAINS 2011

  • question_answer
    Silencing of mRNA has been used in producing transgenic plants resistant to

    A)  bollworms         

    B)  nematodes

    C)  white rusts      

    D)  bacterial blights

    Correct Answer: B

    Solution :

    RNA interference (RNAi) takes place in all eukaryotic organisms as a method of cellular defense. This method involves silencing of a specific mRNA due to a complementary RNA molecule that binds to and prevents translation of the mRNA (silencing). The source of this complementary RNA could be from an infection by viruses having RNA genome or mobile genetic elements (transposons) that replicate via an RNA intermediate. Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant. The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells. These two RNA's being complementary to each other formed, a ds RNA that initiated RNAi and thus, silenced the specific mRNA of the nematode. The result was that the parasite could not survive in a transgenic host expressing specific interfering RNA. The transgenic plant, therefore, got itself protected from the parasite.


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