Answer:
Melvin Calvin used Chlorella
as an experimental material and discovered the first stable compound of photosynthesis,
i.e., 3 phosphoglyceric acid so as to trace the path of carbon by using a
radioactive isotope of carbon and
autoradiography technique.
He then, compared
the radioactive compounds on the chromatogram as a result of which he found and
concluded that the PGA (phosphoglyceric acid), as the first stable product of photosynthesis
and gradually the other sugars including hexoses, tetroses and pentoses etc.
Thus, he
derived the pathway of fixation
from these radioactive products (sugars) formed.
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