| Take a clean glass slide and put few drops of water on it. Now place a complete Rheo leaf on water droplets and examine the cells of leaf under the high power of compound microscope. Put a few drops of concentrated salt/sugar solution on the mounted Rheo leaf on the glass slide. Wait for few minutes and again observe the leaf under the high power of microscope. |
| What will be your observation after few minutes? |
A) Cell contents are separated from the cell wall.
B) Cytoplasm along with plasma membrane has come to lie on one side of cell wall.
C) A clear space is seen between the cell wall and protoplast of the cells.
D) All of these
Correct Answer: D
Solution :
Shrinkage of the protoplast of a plant cell from its cell wall under the influence of a hypertonic solution is called plasmolysis. If we put a Rheo leaf in a concentrated salt/ sugar solution, it will cause exosmosis or withdrawal of water from cytoplasm and then the central vacuole of cell. As a result, the size of cytoplasm as well as central vacuole decreases and hence protoplast reduces. The plasma membrane gets withdrawn from the cell wall and a clear space is seen between the cell wall and protoplast of the cells.
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