| Column-I | Column-II | ||
| A. | Eco RI | I. | Bacillus amyloliquefaciens |
| B. | Bam HI | II. | Haemophilus influenza |
| C. | Hind III | III. | Escherichia coli |
| D. | pBR 322 | IV. | Artificial plasmid |
A) A-III; B-I; C-II; D-IV
B) A-II; B-I; C-IV; D-III
C) A-IV; B-I; C-III; D-II
D) A-I; B-IV; C-II; D-III
Correct Answer: A
Solution :
[a] A: Eco RI is an endonuclease enzyme isolated from strains of E. coli, and is part of the restriction modification system. B : Barn HI (from Bacillus amyloliquefaciens) is a type II restriction endonuclease having the capacity for recognising short sequences of DNA and specifically cleaving them at a target site. C: Hind III is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor \[M{{g}^{2+}}\]via hydrolysis. D: pBR 322 is a plasmid and was one of the first widely used E. coli cloning vectors.
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