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NEET Biology Biotechnology : Principles And Processes Question Bank Self Evaluation Test - Biotechnology: Principles and Processes

  • question_answer
    Temperature required for various steps of polymerase chain reaction (i), (ii) and (iii) are:
    (i)   Denaturation                        (ii) Annealing         
    (iii) Extension

    A) (i) \[-94{}^\circ C\], (ii) \[-60{}^\circ C\], (iii) \[-72{}^\circ C\]

    B) (i)\[-40{}^\circ C\], (ii) \[-72{}^\circ C\], (iii) \[-94{}^\circ C\]

    C) (i) \[-72{}^\circ C\] (ii) - 94°C, (iii) \[-40{}^\circ C\]

    D) (i) \[-90{}^\circ C\], (ii) \[-72{}^\circ C\], (iii) \[-40{}^\circ C\]

    Correct Answer: A

    Solution :

    [a] (i) Denaturation \[-94{}^\circ C\], (ii) Annealing \[-60{}^\circ C\], (iii) Extesnsion \[-72{}^\circ C\]


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