(i) The first recombinant DNA was constructed by using a piece of DNA from a plasmid carrying antibiotic resistance gene in the bacterium Salmonella typhimurium and linked it to the plasmid of E. coli. |
(ii) Cohen and Boyer are known as father of genetic engineering. |
(iii) When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together using DNA ligases. |
(iv) Endonucleases remove nucleotides from the ends of the DNA whereas exonucleases make cuts at specific positions within the |
DNA. |
(v) Presence of more than one recognition sites within the vector will generate several fragments which will complicate the gene cloning. |
(vi) Humulin was the first recombinant DNA based product, produced and marketed in India. |
(vii) YAC vectors contain the telomeric sequence, the centromere and autonomously replicating sequence from yeast chromosomes. |
(viii)Alkaline phosphatase, is used to prevent unwanted self ligation of the vector DNA molecules in procedures of rDNA technology. |
(ix) pBR 322 vector was the first artificial ideal vector constructed by Boliver and Rodriguez. |
(x) Plasmid DNA is coated with histone proteins and can act as genetic factor. |
A) (ii), (iv), (vi) and (x)
B) (ii), (iv), (v) and (x)
C) (i), (iii), (v), (vii), (viii) and (ix)
D) (i), (ii), (iv), (vi) and (x)
Correct Answer: C
You need to login to perform this action.
You will be redirected in
3 sec