NEET Biology Molecular basis of Inheritance / विरासत का आणविक आधार Question Bank Self Evaluation Test - Molecular Basis of Inheritance

  • question_answer
    If a nucleotide lacking a hydroxyl group at the 3' end is added to a PCR, what would be the outcome?        

    A) No additional nucleotides would be added to a growing strand containing that nucleotide.

    B) Strand elongation would proceed as normal.

    C) Nucleotides would only be added at the 5' end.

    D) T. aquaticus DNA polymerase would be denatured.

    Correct Answer: A

    Solution :

    [a] A hydroxyl group at the 3' position of a nucleotide is necessary for the binding of any additional nucleotides. If this hydroxyl group were absent, no other nucleotides could be added to a growing strand.


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