12th Class Biology Sample Paper Biology - Sample Paper-5

  • question_answer
    Explain gel electrophoresis. What are the steps involved in gel electrophoresis?

    Answer:

    Gel electrophoresis is a process for separating molecules by forcing them to migrate through a gel under the influence of an electric field.
    The steps involved in gel electrophoresis are -
    (a) A gel of agarose or polyacrylamide is prepared (of specific porosity).
    (b) DNA fragments of different lengths are allowed to move on gel in an electric field where DNA fragments separate on the basis of size.
    (c) It is 2-D technique to separate DNA fragments of a digest by endonuclease.
    (d) DNA moves in buffer solution according to their molecular weight and size.


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