(i) EcoRI is a well - known restriction endonuclease. How is it named so? Give details. |
(ii) Why is it essential to have a selectable marker in a cloning vector? |
Answer:
(i) EcoRI is derived from Escherichia coli RY13. It is named as follows · The capital letter E is derived from Escherichia (genus). · The letters 'co' are derived from coli (species). · The letter R is derived from RY13 (strain). · The Roman number I indicates that it was the first enzyme isolated from the bacterium E. Coli RY 13. (ii) It is essential to have a selectable marker in the cloning vector because it helps in identifying and eliminating non - transformants and selectively permitting the growth of transformants.
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