question_answer

(i) Name the technique used for the separation of DNA fragments.

(ii) Write the type of matrix used in this technique.

(iii) How is the prepared DNA visualised and extracted for use in recombinant technology?

Answer:

(i) DNA fragments can be separated by the technique called gel electrophoresis.

(ii) The most commonly used matrix is agarose gel, which is a natural polymer extracted from sea weeds.

(iii) A compound called Ethidium Bromide (EtBr) stains DNA, which on exposure with ultraviolet radiationals gives orange light. Hence, DNA fragments appear as orange bands.