question_answer

A bacterial cell is \[la{{c}^{-}}\]\[ga{{l}^{+}}\] and contains F plasmid that is \[la{{c}^{+}}\]. The plasmid has temperature sensitive mutation in its replication system such that above \[40{}^\circ C\] the replication is blocked. A few statements are made about the growth of these organisms in different conditions.

(I)  the cells will grow normally in media containing galactose as the sole source of carbon and incubation temperature as \[42{}^\circ C.\]

(II) The cells will grow normally only if media contains both galactose and lactose irrespective of temperature.

(III) The cells will grow normally in media containing lactose as the sole source of carbon and incubation temperature of \[42{}^\circ C.\]

(IV) If the cells are grown at \[37{}^\circ C.\], lactose should be added to the medium and if the cells are grown at \[42{}^\circ C\], galactose should be added to the medium.

Select the option with false statements.

A) (II) and (III)

B) (I) and (IV)

C) (I), (II) and (IV)

D) (I) and (IV)