| The gene map of a plasmid, which has been used for genetic engineering is shown below. It contains two antibiotic resistance genes. The positions of several restriction endonuclease binding sites are also shown below |
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| Which enzyme(s) would be the most suitable for the cleavage of the plasmid and the DNA containing the gene of interest? |
A) Barn HI
B) BgI
C) Hae III
D) Bam HI and Hae III
Correct Answer: A
Solution :
[a] A suitable restriction endonuclease should cut the plasmid at only one position in order to allow the DNA containing the gene of interest to be inserted into the circular plasmid without loss of any part of the plasmid. Thus, in the map of the plasmid given, the most suitable enzyme will be Bam HI.
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