question_answer

Identify the correct statement.

[A] The first recombinant DNA was constructed by using a piece of DNA from a plasmid carrying antibiotic resistance gene in the bacterium Salmo \[typhimurium\] and linked it to the plasmid of E.coli.

[B] Cohen and Boyer are known as Father of genetic engineering.

[C] When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together using DNA ligase.

[D] Endonucleases remove nucleotides from the ends of the DNA whereas exonucleases make cuts at specific positions within the DNA.

[E] Presence of more than one recognition sites within the vector will generate several fragments which will complicate the gene cloning.

[F] Humulin was the first recombinant DNA based product, produced and marketed

[G] YAC vectors contain the telomeric sequence, the centromere and autonomously replicating sequence yeast chromosomes.

[H] Alkaline phosphatase is used to prevent unwanted self-ligation of the vector DNA molecules in procedures of DNA technology

[I] \[pBR322\] vector was the first artificial ideal vector constructed by Bolivar and Rodriguez

[J] Plasmid DNA is coated with histone proteins and can act as genetic factor.

A) [B], [D], [F] and [J]

B) [B], [D], [E] and [J]

C) [A], [C], [F], [G], [H] and [I]

D) [A], [B], [D], [F] and [J]