Direction for: In each of the following questions a statement of Assertion is given followed by a corresponding statement of Reason just below it. Of the statements mark the correct answer as
Assertion Recognition site should be preferably single and responsive to commonly used restriction enzyme. Reason In pBR 322 alien DNA is ligated generally in the area of Bam-HI site of tetracycline resistance gene.A) If both Assertion and Reason are true and Reason is the correct explanation of the Assertion.
B) If both Assertion and Reason are true but the Reason is not the correct explanation of Assertion.
C) If Assertion is true but Reason is false.
D) If both Assertion and Reason are false
Correct Answer: B
Solution :
Recognition site or site where alien DNA can be inserted should be single because occurrence of more than one recognition site will cause DNA fragmentation and disturb gene cloning.
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