NEET AIPMT SOLVED PAPER 2002

  • question_answer
                    Manipulation of DNA in genetic engineering became possible due to the discovery of:  

    A)                 restriction endonuclease                             

    B)                 DNA ligase

    C)                 transcriptase                                     

    D)                 primase

    Correct Answer: A

    Solution :

                    Isolation of restriction endonucleases by Nathans and Smith (1970) made it possible to cut DNA at specific sites. Restriction enzyme can cut both strands of DNA when foreign nucleotides arc introduced in the cell. They cleave DNA to generate a nick with a 5' phosphoryl and 3' hydroxyl terminus.


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