A) denaturation and annealing
B) annealing and extension
C) extension and amplification
D) None of the above
Correct Answer: B
Solution :
The process of rDNA technology involve following process; isolation of DNA molecule, cutting of DNA molecule, cutting of DNA at specific site amplification of gene using PCR, insertion of recombinant DNA into host cells obtain foreign gene product then down stream processing. During amplification of gene using PCR Taq DNA polymerase isolated from thermophilic bacterium, Themus acquaticus is used between extension (final step) and annealing (joining II step).
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