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BHU PMT BHU PMT (Screening) Solved Paper-2010

  • question_answer
    The linking of antibiotic resistance gene with the plasmid vector became possible with

    A)  DNA ligase                        

    B)  endonucleases

    C)  DNA polymerase            

    D)  exonucleases

    Correct Answer: A

    Solution :

                     DNA ligase is used to seal the nicks that remains in recombinant DNA molecule. Intact, DNA ligase joins  together the  neighbouring nucleotides flanking a discontinuity in a DNA strand by forming a phosphodiester bond. A recombinant DNA molecule is a vector into which the desired DNA fragment has been inserted to enable its cloning in an appropriate host,


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