CET Karnataka Medical CET - Karnataka Medical Solved Paper-2001

  • question_answer
    The test used for identifying peptide linkage in proteins is:

    A)  Borsches test

    B)  Molischs test

    C)  Ninhydrin test

    D)  Biuret test

    Correct Answer: D

    Solution :

    For the identification of peptide linkage the test performed is Biuret test. \[N{{H}_{2}}CON{{H}_{2}}\xrightarrow{Heat}N{{H}_{3}}+NH=C=O\] \[\underset{Peptide\,bond}{\mathop{NH=C=O}}\,+N{{H}_{2}}CON{{H}_{2}}\xrightarrow{{}}\] \[\underset{Biuret}{\mathop{{{H}_{2}}NCONHCON{{H}_{2}}}}\,\]


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