A) restriction endonucleases and topoisomerases
B) endonucleases and polymerases
C) restriction endonucleases and ligases
D) peptidases and ligases.
Correct Answer: C
Solution :
: An important step in the process of genetic engineering is the preparation of recombinant DNA (r DNA). For this, the vector DNA and foreign DNA (carrying gene of interest) are needed to be cut at specific sites and joined to producer DNA. This is achieved by enzymes, restriction endonucleases and DNA ligases. Restriction endonucleases act as molecular scissors or chemical scalpels which recognize the base sequence at palindrome sites in DNA duplex (a section of ds DNA having sequence of bases on one strand inverted and repeated on the other) and cut each of the two strands of the double ] helix at specific points in their sugar phosphate backbones. DNA ligases are known as genetic gum as they form phosphodiester bonds between adjacent nucleotides and covaiently link two individual fragments of ds DNA.
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