A) Denaturation of DNA on gel is hybridization with specific probe
B) Production of a group of genetically identical cells
C) Digestion of DNA by restriction enzyme
D) Denaturation of DNA from a nucleated cell such as the one from the scene of crime.
E) None of the above
Correct Answer: C
Solution :
First step in the Southern Blot Technique is the digestion of DNA by restriction enzyme. \[\downarrow \] Restriction fragment preparation \[\downarrow \] Separation by electrophoresis \[\downarrow \] Denaturation of DNA by chemical treatment \[\downarrow \] Single-stranded DNA are transferred on to special paper or nylon \[\downarrow \] Use of radioactive probe \[\downarrow \] Autoradiography
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