DUMET Medical DUMET Medical Solved Paper-2005

  • question_answer
    The function of polymerase chain reaction (PCR) is :

    A)  translation

    B)  transduction

    C)  DNA amplification

    D)  none of the above

    Correct Answer: C

    Solution :

     Amplification of DNA takes place through polymerase chain reaction (PCR). PCR was discovered by Kary Mullis (1985). Taq DNA polymerase enzyme is used in PCR which can act at \[{{75}^{o}}C\]. The denaturation of DNA takes place at \[90-{{80}^{o}}C\] and renaturation at\[40-{{60}^{o}}C\]. Translation is the synthesis of protein on ribosomes by m-RNA, t-RNA and amino acids. Transduction is the transfer of bacteria! DNA from the donor to recepient cells through bacteriophages.


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