A) protoplast isolation
B) cell culture preparation
C) protoplast fusion
D) hardening
Correct Answer: C
Solution :
Somatic hybridization involves the fusion of protoplasts of two different species which resulted in hybrid. Naked protoplasts are obtained by dissolution of their cell walls by the mecerating enzymes such as pectinase and cellulase. Fusion of protoplasts from two different varieties can be enhanced by treating with polyethylene glycol (PEG) in presence of high voltage electric current.
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