DUMET Medical DUMET Medical Solved Paper-2011

  • question_answer
    ELISA assay

    A)  uses complement mediated cell lysis

    B)  uses a radiolabelled second antibody

    C)  involves addition of substrate which is convened into coloured end product

    D)  requires red blood cells

    Correct Answer: C

    Solution :

    In ELISA (Enzyme Linked Immunosorbent Assay), the antibody is bound to an inert polymer support than exposed to the sample. The second antibody used is one that has an enzyme attached to it that converts a colourless or nonfluorescent substrate into a coloured or fluorescent product.


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