DUMET Medical DUMET Medical Solved Paper-2011

  • question_answer
    PCR proceeds in three distinct steps governed by temperature, they are in order of

    A)  denaturation, annealing, synthesis

    B)  synthesis, annealing, denaturation

    C)  annealing, synthesis, denaturation

    D)  denaturation, synthesis, annealing

    Correct Answer: A

    Solution :

    Poiymerase chain reaction was developed by Kary Multis. It is composed of denaturation of DNA at high temperature, annealing of primer at the ends of DNA strands and synthesis/polymerizaton by Taq polymerase.


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