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Manipal Medical Manipal Medical Solved Paper-2015

  • question_answer
    Some of the common steps involved in the production of humulin are given below. Choose the correct sequence. (I) Synthesis of gene (DNA) for human insulin artificially. (II) Culturing of recombinant E. coli in bacteria. (III) Purification of humulin. (IV) Insertion of human insulin gene in plasmid DNA. (V) Introduction of recombinant plasmid into E. coli. (VI) Extraction of recombinant gene product from E. coli.

    A) II, I, IV, III, V, VI         

    B) I, III, V, VI, II, IV

    C) I, IV, V, II, VI, III                   

    D) III, V, II, I, VI, IV

    Correct Answer: B

    Solution :

    (i) Synthesis of gene (DNA) from human insulin artificially. (iv) Insertion of human insulin gene in plasmid DNA. (v) ntroduction of recombinant plasmid into E. coli. (ii) Culturing of recombinant E.coli in bacteria. (vi) Extraction of recombinant gene product from E. coli. (iii) Purification of humulin.


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