A) Restriction endonuclease ? Production of DNA fragments from gene cloning.
B) DNA ligase ? Enzyme that cuts DNA producing sticky ends
C) DNA polymerase ? Copies DNA sequences in the PCR
D) Reverse transcriptase ? Production of c DNA from mRNA
Correct Answer: B
Solution :
DNA ligase is the molecular glue in RDT. It joins the fragment together by addition of required nucleotides like A, T, G and C.
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