WB JEE Medical WB JEE Medical Solved Paper-2014

  • question_answer
    During gene cloning, the enzyme used to join the insert DNA with the plasmid vector is

    A)  DNA ligase

    B)  restriction endonuclease

    C)  alkaline phosphatase

    D)  exonuclease

    Correct Answer: A

    Solution :

     In molecular biology, DNA ligase is a special type of enzyme that is used to seal the double  stranded  breaks   {i.e., breaks  in  both complementary strands of DNA) or is used to join insert DNA with the plasmid vector. DNA ligase has its applications in DNA replication and DNA repair. Hence, it is also known as molecular glue. While the other options given, i.e., restriction endonuclease are used to make cuts at specific position within DNA, exonuclease are used remove nucleotides from the ends of DNA (either 5 or 3) in one strand of duplex and alkaline phosphatase are used to responsible for removing phosphate groups from nucleotides, proteins, etc.


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